



Individual Submission                                            K. Tsoi
Internet-Draft                                             Aevum Network
Intended status: Informational                               15 May 2026
Expires: 16 November 2026


 Iron Triangle Trace for Longevity (ITT-L): A PACR Application Payload
                  Format for Gene-Level Longevity Data
                          draft-aevum-itt-l-00

Abstract

   This document defines Iron Triangle Trace for Longevity (ITT-L), a
   domain-specific Application Payload Format carried within the PACR
   (Physical and Causal Auditable Record) envelope.  Each ITT-L record
   binds four measurement facets — gene, mechanism, drug/metabolite, and
   epigenetic — into a single independently-citable asset with causal
   predecessor chain (Π) integrity provided by the PACR transport layer.
   ITT-L is NOT a standalone protocol; it is a payload format that
   leverages PACR for physical proof (Λ, Landauer cost) and causal
   ordering (Π chain), and AgentCard for service identity.

Status of This Memo

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   provisions of BCP 78 and BCP 79.

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   This Internet-Draft will expire on 16 November 2026.

Copyright Notice

   Copyright (c) 2026 IETF Trust and the persons identified as the
   document authors.  All rights reserved.








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   This document is subject to BCP 78 and the IETF Trust's Legal
   Provisions Relating to IETF Documents (https://trustee.ietf.org/
   license-info) in effect on the date of publication of this document.
   Please review these documents carefully, as they describe your rights
   and restrictions with respect to this document.

Table of Contents

   1.  Introduction  . . . . . . . . . . . . . . . . . . . . . . . .   2
   2.  Terminology . . . . . . . . . . . . . . . . . . . . . . . . .   3
   3.  Payload Structure . . . . . . . . . . . . . . . . . . . . . .   3
     3.1.  Required Fields . . . . . . . . . . . . . . . . . . . . .   3
     3.2.  Optional Fields . . . . . . . . . . . . . . . . . . . . .   4
   4.  Coupling Edges  . . . . . . . . . . . . . . . . . . . . . . .   4
   5.  Falsifiability Considerations . . . . . . . . . . . . . . . .   5
   6.  Security Considerations . . . . . . . . . . . . . . . . . . .   5
   7.  IANA Considerations . . . . . . . . . . . . . . . . . . . . .   5
   8.  Normative References  . . . . . . . . . . . . . . . . . . . .   6
   9.  Informative References  . . . . . . . . . . . . . . . . . . .   6
   Author's Address  . . . . . . . . . . . . . . . . . . . . . . . .   7

1.  Introduction

   Longevity research produces data across multiple measurement layers:
   genomic variants, proteomic drug targets, metabolic pathway
   participants, and epigenetic clock markers.  These data are typically
   stored in heterogeneous databases with no common citation format,
   making cross-layer validation and third-party reproducibility
   difficult.

   ITT-L addresses this by defining a standardized JSON payload format
   that binds all four measurement layers per gene into a single record.
   Each ITT-L record is carried inside a PACR envelope, which provides:

   *  *ι* (CausalId): 128-bit unique record identity

   *  *Π* (predecessor chain): causal ordering of DoD validation steps

   *  *Λ* (Landauer cost): physical proof that record generation
      consumed real computational resources

   *  *Ω* (resource triple): energy, time, and space consumed

   *  *Γ* (cognitive split): statistical complexity vs.  entropy rate
      (S_T/H_T)






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   ITT-L is complementary to [I-D.draft-aevum-agentcard], which declares
   agent identity and capabilities, and
   [I-D.draft-aevum-causal-intervention-record], which defines the PACR
   transport envelope.

2.  Terminology

   The key words "MUST", "MUST NOT", "REQUIRED", "SHALL", "SHALL NOT",
   "SHOULD", "SHOULD NOT", "RECOMMENDED", "NOT RECOMMENDED", "MAY", and
   "OPTIONAL" in this document are to be interpreted as described in BCP
   14 [RFC2119] [RFC8174] when, and only when, they appear in all
   capitals, as shown here.

   ITT-L Record:  One Iron Triangle Trace for Longevity payload, carried
      inside the body(P) field of a PACR envelope.

   Facet:  One of the four measurement dimensions: gene_facet,
      mechanism_facet, drug_metabolite_facet, or epigenetic_facet.

   Coupling Edge:  A measurement of information fidelity between two
      facets (e.g., gene→protein translation fidelity).  May be
      unmeasured (honest marker).

   H_ITT_gene:  A composite health index per gene, ∈ [0,1], computed as
      the product of facet completeness, facet consistency, and edge
      factor.

   DoD (Definition of Done):  A 7-condition validation gate (D1-D7)
      applied to each gene before ITT-L record generation.

3.  Payload Structure

   An ITT-L payload is a JSON object carried in the body(P) field of a
   PACR Application Payload.  The JSON Schema is defined at
   https://eon.aevum.network/itt-l-schema-v1.json.

3.1.  Required Fields

   The following fields MUST be present in every ITT-L payload:

   *  record_type: MUST be the string "iron_triangle_trace_l".
   *  schema_version: MUST be "1.0".
   *  gene_facet: object with gene_symbol (string) and causal_score
      (number).
   *  mechanism_facet: object with KEGG/Reactome pathway data and a
      mechanism_partial boolean (honest marker).
   *  drug_metabolite_facet: object with DrugBank entries, AlphaFold
      pLDDT, and metabolites.



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   *  coupling_edges: object with three sub-objects (gene_protein,
      protein_epi, epi_gene), each containing value (number or null),
      status (enum: unmeasured, estimated, measured,
      blocked_external_data), and source (string or null).
   *  h_itt_gene: number ∈ [0,1], computed as facet_completeness ×
      facet_consistency × (1 + edges_measured/3) / 2.  This formula is
      frozen in v1.0.

3.2.  Optional Fields

   The following fields MAY be present:

   *  epigenetic_facet: object with EPI-score, Horvath CpG count, and
      epigenetic modifier status.
   *  discovery_class: enum "novel" | "drug_ready", indicating the DoD
      track that produced this record.
   *  auto_discovered: boolean indicating whether the record was
      generated by the ITT-grower daemon rather than human curation.

4.  Coupling Edges

   The three coupling edges map to the iron triangle framework described
   in [I-D.veneter]: genome, proteome, and epigenome form three
   vertices; the edges measure information flow fidelity between them.

   gene_protein:  Translation fidelity — mRNA-to-protein correlation.
      Measurement operator: Ribosome Profiling + proteomics Pearson r
      (Frobenius norm).

   protein_epi:  Modification fidelity — SIRT1 activity × DNMT3A
      methylation maintenance rate, normalized to [0,1].

   epi_gene:  Regulatory fidelity — ATAC-seq open chromatin peak overlap
      at FOXO3 binding sites.

   In ITT-L v1.0, all coupling edges default to status "unmeasured" —
   this is an honest marker, not a data gap.  Edges transition to
   "measured" when the corresponding measurement operator produces a
   value.  The edge_factor component of H_ITT_gene increases from 0.5
   (all unmeasured) toward 1.0 (all measured), providing a built-in
   incentive for edge measurement without penalizing records that
   legitimately lack measurement data.









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5.  Falsifiability Considerations

   ITT-L is designed as an engineering protocol that also supports
   scientific falsifiability.  Four falsifiable predictions are defined
   in the companion Falsifiability Manifest (EON-ITT-L-FALSIFIABILITY-
   MANIFEST.md):

   1.  *F1 (Intervention Effect)*: novel 6/6 genes show Horvath Δage ≤
       -3 years after intervention.  Falsified if ≥30 wet-lab records
       show otherwise.
   2.  *F2 (Coupling Prediction)*: high protein_epi genes show greater
       intervention ROI (r ≥ 0.2).  Falsified if correlation is below
       threshold.
   3.  *F3 (H_ITT Predictive Power)*: genes with h_itt_gene ≥ 0.7 are
       adopted as clinical trial targets at a higher rate than baseline.
       Falsified if p ≥ 0.05.
   4.  *F4 (Facet Completeness)*: complete 4-facet genes show higher D7
       wet-lab pass rates than incomplete genes.  Falsified if p ≥ 0.05.

6.  Security Considerations

   ITT-L does not introduce new security considerations beyond those
   already described in the PACR transport specification
   [I-D.draft-aevum-causal-intervention-record].  The following
   considerations apply:

   *  Λ (Landauer cost) provides a physical lower bound on forgery cost
      — any attacker attempting to inject fraudulent ITT-L records MUST
      spend at least LANDAUER_JOULES_300K (≈2.87 × 10⁻²¹ J) per bit of
      erased information.  While individually small, this cost scales
      linearly with record size and count, making bulk forgery
      detectable through energy anomaly.

   *  The Π (predecessor) chain ensures that any ITT-L record's causal
      ancestry can be independently verified without trusting the EON
      service.

   *  Sensitive gene data (e.g., rare variant information) is public
      research data derived from UK Biobank, GTEx, and PubMed.  No
      personally identifiable information is stored in ITT-L records.

7.  IANA Considerations

   This document requests the registration of the following media type:

   Type name:  application

   Subtype name:  eon-itt-l+json



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   Required parameters:  N/A

   Optional parameters:  N/A

   Encoding considerations:  binary (UTF-8 JSON)

   Security considerations:  See Security Considerations (Section 6) of
      this document.

   Interoperability considerations:  Payload MUST validate against the
      JSON Schema at https://eon.aevum.network/itt-l-schema-v1.json

   Published specification:  This document

   Applications that use this media type:  EON Longevity Target
      Discovery system

   Fragment identifier considerations:  N/A

   Restrictions on usage:  N/A

   Provisional registration:  Yes

8.  Normative References

   [RFC2119]  Bradner, S., "Key words for use in RFCs to Indicate
              Requirement Levels", March 1997,
              <https://www.rfc-editor.org/info/rfc2119>.

   [RFC8174]  Leiba, B., "Ambiguity of Uppercase vs Lowercase in RFC
              2119 Key Words", May 2017,
              <https://www.rfc-editor.org/info/rfc8174>.

9.  Informative References

   [I-D.draft-aevum-agentcard]
              Tsoi, K., "AgentCard: A Framework-Neutral Identity and
              Capability Declaration Format", 23 April 2026,
              <https://datatracker.ietf.org/doc/draft-aevum-agentcard/>.

   [I-D.draft-aevum-causal-intervention-record]
              Tsoi, K., "Causal Intervention Record (PACR): A Physics-
              Grounded Audit Trail for Agent Actions", 26 April 2026,
              <https://datatracker.ietf.org/doc/draft-aevum-causal-
              intervention-record/>.






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   [I-D.veneter]
              Venter, C., "Iron Triangle of Longevity Research", April
              2026, <https://github.com/kwailapt/EON>.

Author's Address

   Kwai Lap Tsoi
   Aevum Network
   Email: kwailapt@gmail.com
   URI:   https://github.com/kwailapt/EON









































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